Product Name	DGKK
Chinese Name	甘油二酯激酶κ/DGK-κ抗体
Alias	142 kDa diacylglycerol kinase; DAG kinase kappa; DGK kappa; DGKK_HUMAN; Diacylglycerol kinase kappa; Diglyceride kinase kappa.
Research Area	Tumour Cell biology Signal transduction The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat(predicted:Human,Mouse)
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	142kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DGKK: 181-280/1271
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011] Function: DGKK (Diacylglycerol kinase kappa) phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Subunit: Does not form homooligomers. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein Tissue Specificity: Expressed in testis, and to a lesser extent in placenta. Post-translational modifications: Phosphorylated at Tyr-78 by some member of the SRC family in response to H(2)O(2). Similarity: Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. SWISS: Q5KSL6 Gene ID: 139189 Database links: Entrez Gene: 139189 Human Entrez Gene: 331374 Mouse SwissProt: Q5KSL6 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGKK) Polyclonal Antibody, Unconjugated (SL14300R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGKK) Polyclonal Antibody, Unconjugated (SL14300R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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