Product Name	DDX49
Chinese Name	ATP依赖RNA解旋酶DDX49抗体
Alias	DEAD (Asp Glu Ala Asp) box polypeptide 49; DEAD box protein 49; DDX49_HUMAN; FLJ10432; Probable ATP dependent RNA helicase DDX49; R27090 2.
Research Area	Cell biology Developmental biology transcriptional regulatory factor Binding protein Cell differentiation Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat(predicted:Human,Mouse,Dog,Pig,Cow,Sheep)
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DDX49: 351-483/483
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis and cellular growth and division. DDX49 (DEAD-box protein 49) is a 483 amino acid protein that contains one helicase ATP-binding domain and one helicase C-terminal domain. One of several members of the DEAD-box protein family, DDX49 may function as a an RNA helicase that is involved in pre-mRNA splicing events. Function: The function of DDX49 has not yet been determined. Similarity: Belongs to the DEAD box helicase family. DDX49/DBP8 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q9Y6V7 Gene ID: 54555 Database links: Entrez Gene: 54555 Human Entrez Gene: 234374 Mouse Entrez Gene: 290660 Rat SwissProt: Q9Y6V7 Human SwissProt: Q3UTV0 Mouse SwissProt: Q4FZF3 Mouse Unigene: 143187 Human Unigene: 388127 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX49) Polyclonal Antibody, Unconjugated (SL14330R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX49) Polyclonal Antibody, Unconjugated (SL14330R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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