Product Name	RBBP6
Chinese Name	增殖潜能相关蛋白抗体
Alias	Retinoblastoma-binding protein 6; proliferation potential protein related; p53-associated cellular protein of testis; Proliferation potential-related protein; Retinoblastoma-binding Q protein 1; Protein RBQ-1; My038; PACT; Protein P2P-R; RBBP6_HUMAN; P2P-R.
Research Area	Tumour Cell biology Developmental biology Signal transduction Cyclin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse(predicted:Human,Rat)
Applications	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	201kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RBBP6: 2-150/1792
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	he retinoblastoma tumor suppressor (pRB) protein binds with many other proteins. In various human cancers, pRB suppresses cellular proliferation and is inactivated. Cell cycle-dependent phosphorylation regulates the activity of pRB. This gene encodes a protein which binds to underphosphorylated but not phosphorylated pRB. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth. Subunit: Interacts with p53/TP53 and RB1. Interacts also with MDM2 and YBX1. Interacts with NEK6. Subcellular Location: Nucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, centrosome. Note=Colocalizes with mitotic chromosomes. Co-localizes with NEK6 in the centrosome. Tissue Specificity: Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer. Post-translational modifications: Phosphorylated by NEK6. Similarity: Contains 1 CCHC-type zinc finger. Contains 1 DWNN domain. Contains 1 RING-type zinc finger. SWISS: Q7Z6E9 Gene ID: 5930 Database links: Entrez Gene: 5930 Human Entrez Gene: 19647 Mouse Entrez Gene: 308968 Rat Omim: 600938 Human SwissProt: Q7Z6E9 Human SwissProt: P97868 Mouse Unigene: 188553 Human Unigene: 4480 Mouse 信号传导（Signaling Intermediates） P2P-P又称PACT即P53相关细胞蛋白（P53 associated cellular protein-testes derived）， P2P-P蛋白是p53的负调控因子，而且在细胞生长和胚胎发育，Cell differentiation、前体mRNA剪接、周期阻滞、凋亡过程中起到了重要作用。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RBBP6) Polyclonal Antibody, Unconjugated (SL1483R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.