Product Name	CH25H
Chinese Name	胆固醇25羟化酶抗体
Alias	C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN.
literatures	Specific References (4) | SL6480R has been referenced in 4 publications. [IF=31.25] Ceglia, Simona. et al. An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cells. NAT IMMUNOL. 2023 Jan;:1-14 WB ; Mouse. PubMed:36658240 [IF=7.971] Samantha A. Hutchinson. et al. Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer. Oncogene. 2021 Mar;:1-12 IHC ; Human. PubMed:33742124 [IF=5.56] Mukherjee, Pallavi, et al. "Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-β and Cholesterol 25-hydroxylase in Jejunum." Journal of Lipid Research (2017): jlr-M076554. IHC-P ; Mouse. PubMed:28592401 [IF=4.122] Chattopadhyay et al. Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung Cancer. (2018) Sci.Rep. 8:9032 IHC ; mouse. PubMed:29899427
Research Area	Tumour Cell biology Signal transduction Kinases and Phosphatases The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,Rat
Applications	WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CH25H: 1-100/272
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. Function: CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. Subcellular Location: Endoplasmic reticulum membrane Post-translational modifications: N-glycosylated. Similarity: Belongs to the sterol desaturase family. SWISS: O95992 Gene ID: 9023 Database links: Entrez Gene: 9023 Human Entrez Gene: 12642 Mouse Entrez Gene: 309527 Rat Omim: 604551 Human SwissProt: O95992 Human SwissProt: Q9Z0F5 Mouse SwissProt: Q4QQV7 Rat
Product Picture	Figure 1. Sample: Lane 1: Recombinant human CH25H protein, His(SL41322P) Primary: Anti-CH25H (SL6480R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: kDa Observed band size: 11 kDa Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CH25H Polyclonal Antibody, Unconjugated (SL6480R) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining