Product Name	Di-Methyl-Histone H3 (Lys23)
Chinese Name
Product Type	Methylated anti
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	C11G10
React Species	Human(predicted:Mouse,Rat,Monkey)
Applications	WB (1:500-2000), IHC-P (1:200-1000), IHC-F (1:200-1000), IF (1:200-1000), ICC/IF (1:100-250), IP (1:20-50), ChIP (6 ug/5x106 cells) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
Lsotype	IgG
Purification	Antigen affinity purification
Buffer Solution	1M TBS(pH7.4) with 1% BSA, 3% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Histone post-translational modifications (PTMs) are a key mechanism by which epigenetics regulates chromatin structure and are known as the "histone code". Post-translational modifications on histones include acetylation, methylation, phosphorylation and some novel acylation modifications discovered in recent years. These histone modifications directly affect the binding of chromatin to transcription factors or other epigenetic regulators, and change genome stability and gene transcription, etc. Histone methylation usually occurs on lysine and arginine residues of core histones. Histone methylation can either promote or inhibit gene transcription, depending on whether histone methylation occurs on lysine or arginine and the number of methyl groups (lysine can undergo mono-, di- and tri-methylation, and arginine can undergo mono-, symmetric and asymmetric di-methylation). Histone lysine methylation usually occurs on lysine 4, 9, 27, 36 and 79 of histone H3 and lysine 20 of histone H4; arginine methylation usually occurs on arginine 2, 8, 17 and 26 of histone H3 and arginine 3 of histone H4. Protein methyltransferases (HMT) and histone demethylases (HDM) are the main regulatory factors of methylation modifications.