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Product Name Histone H4 (NT) Chinese Name Immunogen Species Mouse Clonality Monoclonal Clone NO. D12G5 React Species (predicted: Human, Mouse, Rat, Monkey, ) Applications WB=1:500-1:2000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 11kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml Lsotype IgG Purification Antigen affinity purification Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail 核小体由四个核心组蛋白(H2A、H2B、H3和H4)组成,是染色质的主要组成部分。核小体将DNA包裹并压缩成染色质,限制DNA进入细胞机制。组蛋白H4是参与真核细胞染色质结构的五种主要组蛋白之一。H4具有一个主要的球状结构域和一个长的N末端尾巴,它参与了“串珠”组织的核小体结构。组蛋白是高度翻译后修饰的。共价键合修饰包括N端尾部的乙酰化和甲基化。这些修饰可能改变位于与其亲本组蛋白八聚体相关的DNA上的基因的表达。 Product Picture Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: MCF-7, BRL, N2a, COS-7, Mouse liver
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 11 kDa
Observed MW: 11 kDa
Tissue: Human neuroblastoma
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:500
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit(Mouse)(sp-0024)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for SLM-60214M
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