(This product is 10X mother solution, 1X is recommended for working concentration)

 

In the in vitro culture of tissue cells and the dispersion of tissue cells in primary cell culture (preparation of tissue blocks into a single cell suspension) and passage cell culture, the digestion and dispersion of adherent growth cells should use tissue cell digestion solution. Commonly used digestive solutions are trypsin, EDTA, etc., whose main function is to hydrolyze intercellular proteins (such as extracellular matrix), disperse tissues or adherent cells into single cells, and make cell suspensions for further experiments.

The trypsin-EDTA solution (Trypsin-EDTA Solution) produced by Suo Laibao contains 2.5% trypsin 0.2% EDTA (5.3mM), dissolved in a calcium-magnesium-free balanced salt solution, sterilized by filtration, and can be directly used for cultivation Digestion of cells and tissues. This product has the characteristics of convenience, speed, stability and safety, and good cell status. Usually about 2 minutes at room temperature can digest most of the adherent cells. Trypsin-EDTA digestion solution has two types of products with and without phenol red. Phenol red has a pH indicator function.

 

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