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Purpose
Our Canine Herpesvirus(HV) ELISA kit is to assay Herpesvirus(HV) levels in Canine serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to Herpesvirus(HV) . Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for Herpesvirus(HV) is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain Herpesvirus(HV) and HRP conjugated Herpesvirus(HV) antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of Herpesvirus(HV) is determined by comparing with the CUTOFF value.
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