Product overview

Rat High mobility group protein B1,HMGB-1 ELISA Kit (Catalog No. SL0347Ra) is an enzyme-linked immunosorbent assay kit for detection of the target in In serum, plasma, culture media or any biological fluid.

Assay principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to HMGB-1. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for HMGB-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain HMGB-1 and HRP conjugated HMGB-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of HMGB-1. You can calculate the concentration of HMGB-1 in the samples by comparing the OD of the samples to the standard curve. 3rd

Specifications

• Assay range: 10pg/ml-800pg/ml
• Sensitivity: 2.5pg/ml
• Standard: 1080pg/ml
• Sample volume: 10ul
• Wavelength: 450nm
• Size: 96T/48T

Kit components

• User manual
• Closure plate membrane
• Sealed bags
• Pre-coated microelisa stripplate
• Standard (1080 pg/ml)
• Standard diluent
• HRP-conjugate reagent
• Sample diluent
• Chromogen Solution A
• Chromogen Solution B
• Stop Solution

Sample type

In serum, plasma, culture media or any biological fluid.

For research use only

Not for use in diagnostic or therapeutic procedures. Please refer to the instruction manual (PDF) for the complete protocol, sample preparation and calculation of results.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: 3 working days for small quantities; 5-8 working days for larger quantities; 2-4 weeks for special or non-standard items
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)