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Hamster Sendai Virus5 (SV5)ELISA Kit
Hamster Sendai Virus5 (SV5)ELISA Kit
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Details

Purpose

Our Hamster Sendai Virus5(SV5)ELISA kit is to assay SV5 levels in Hamster serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to SV5. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for SV5 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain SV5 and HRP conjugated SV5 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of SV5 is determined by comparing with the CUTOFF value.

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