Measurement Significance: Acetyl coenzyme A is widely present in animals, plants, microorganisms and cultured cells. It is an important intermediate metabolite produced in the process of biometric energy substance metabolism. In vivo energy substance metabolism is a hub material. Sugar, fat, protein three major nutrients via a acetyl coenzyme A converge into a common metabolic pathway - tricarboxylic acid circulation and phosphorylation, and the hydroxide is thoroughly oxidized into carbon dioxide and water, and the release energy is used for ATP synthesis. Further, the acetyl coenzyme a is a precursor substance such as a physically active material such as a synthetic fatty acid, ketone body, cholesterol and derivative thereof.

Measurement principles: Maki-dehydrogenase can catalyze malic acid and NAD to form grassoyacetic acid and NADH. Citric acid synthase can catalyze acetyl co-enzyme A and grassoyacetic acid to form citric acid and coenzyme A. The coupling reaction of malic dehydrogenase and citric acid synthase, acetyl coenzyme a content and NADH were met, and the rise rate of the absorbent value of the absorbent enzyme A was reacted with a higher rate of the acetyl coenzyme A.

Need instruments, supplies: UV spectrophotometric / enzyme label, water bath, desktop centrifuge, adjustable pipette, trace quantine-based cuvette / 96-well plate, mortar, ice and distilled water .

 

 

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