Measurement Significance: NR (EC 1.7.1.3) is widely existing in plants, is a key enzyme that is transformed into ammonia nitrogen in plant nitrate nitrogen, also induce enzyme, and the output of crops.And quality has an impact.

Determination principle: NR catalyzed nitrate was reduced to nitrite, NO3 ˉ + NADH + H + → NO2 ˉ + NAD + + H 2 O; produced nitrite can be in acidic conditions, with pair-aminobenzenesulfurAcid and α - naphthyl amine to form a red azo compound; the resulting red azo compound has the maximum absorption peak at 340 nm and can be determined by spectrophotometry.

Need instruments, supplies: visible spectrophotometer / enzyme label, water bath, desktop centrifuge, adjustable pipette, trace quantine-based cuvette / 96-well plate, mortar, ice and distilled water.

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