Micro NO Content Assay Kit

Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/ microplate reader

Catalog Number: AK0259

Size: 100T/96S

Product Description

Nitric Oxide (NO) is a highly unstable biological free radical with small molecule, simple structure，NO is gas at room temperature, slightly soluble in water and fat-soluble, which can diffuse through biofilms quickly. As a new type of biological messenger molecule, it plays a role in transmitting signals between cells and within cells. It is widely distributed in various tissues, especially neural tissues. It also plays a very important role in the nervous, circulatory, respiratory, digestive, and urogenital systems of the body.   NO is easily oxidized to form NO2- and NO3- in the body or in aqueous solution. This method uses nitrate reductase to reduce NO3- to NO2-  specifically. Under acidic conditions, NO2-  and diazonium sulfonamide produce diazo compounds. The compounds could further coupled with naphthyl vinyl diamine, the product has a characteristic absorption peak at 550 nm, and its absorbance value can be measured to calculate the NO content

 

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