Measurement Significance:

β-Gal (EC 3.2.1.23) is widely present in animals, plants, microorganisms, and cultured cells, catalyzes β galactoside compounds.The medium β galactoside is hydrolyzed, and there is also a function of turning lactant.The β-Gal can not only release the energy stored energy, but also catalyze the hydrolyzate of polysaccharide, glycoprotein, and galactose and galactin and galactose., Release free galactose. As determined: β-gal decomposition pair-nitrobenzene-β-D-pyranium galactoside to-nitrophenol, the latter has the maximum absorption peak at 400 nm, by measuring the absorbanceThe value rise rate is calculated to calculate β-GAL activity.
Self-stock: Visit spectrophotometer, desktop centrifuge, water bath, adjustable pipette, 1ml glass cuvette, mortar, ice and distilled water.

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