Measuring significance: β-xylosidase (EC3.2.1.37) is present in organisms such as plants, bacteria and fungi, is the key to catalyzing chitosan halfcellulose degradationEnzyme, product xylose can be used as a carbon source to microbial fermentation.In addition, β-xyloside can also be used as a biological bleach to the papermaking industry, which has a wide range of application value than traditional bleaching methods.

Measurement principle: β-xyloglycosidase catalyzed to nitrophenol-β-D-xylosidin produces a nitrophenol, and the nitrophenol has a characteristic absorption peak at 405 nm, and the 405 nm light absorption increase rate is measured.The β-xylosidase activity can be calculated.

Required instruments, consumables: balance, low temperature centrifuge, visible spectrophotometer / enzyme symbol, trace quantum-based cuvette / 96-well plate and distilled water.

 

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