Measurement Significance: cellulase is a multi-component enzyme system produced by microorganisms, can hydrolyzate cellulose β-1,4 glucoside bonds to form glucose, and filter enzyme is.A component, studying filter enzyme viability is very important for cellulase research.

Measurement principle: Reducing sugar produced by filter paper enzyme water and 3,5-dinitrophyl salicylic acid into reddish brown amino compound, with maximum light absorption at 540 nm, in a certain range of reactive liquid colorThe amount of depression is proportional to the amount of reducing sugar, and the viability of the filter enzyme can be determined.

Required instruments, supplies: balance, mortar, low temperature centrifuge, visible spectrophotometer / enzyme label, trace quantum-based cuvette / 96-well plate, constant temperature water bath.

 

 

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