Measurement Significance: Fruit 1,6-Dioliocerase (EC4.1.2.13) is an important enzyme in glycolysis and sugar isomatic pathways, catalytic fructose 1,6-di phosphate reversal lysis is a phosphate dihydroxyacetone and 3-phosphorched aldehyde, existing in animal and plant and microorganisms, providing energy for biomerical synthesis metabolism.

 

Measurement principle: Fruit 1,6-diphosphate catalyzed in fructose 1,6-diphosphate to form 3-phospholiol and phosphate hydroxypropone, amtrophhionic enzyme and α-phosphateCatalytic NADH and phosphate hydroxypropanone undergived glycerol dehydrogenase to form NAD and alpha-phosphoric acid glycerol, and the variation of the absorbance value at 340 nm can reflect the high and low of fructose 1,6-diphosphate activity.

Required instruments, consumables: balance, low temperature centrifuge, mortar, ultraviolet spectrometry / enzyme syndrome, trace quantum-British Biocani / 96-well plate (UV board).

 

 

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