Measuring significance: glucose-6-phosphatase (Glucose 6 Phosphat, G6Pase, EC 3.1.3.9) is widely used in animals, plants, microorganisms and cells, is a sugarProcess hydrolysis of glucose-6-phosphoric acid to generate an important role in ensuring blood sugar.

 

Determination principle: G6P catalytic glucose-6-phosphoric acid to generate glucose, cyclonic enzymes and glucoseThe dehydrogenase further catalyzes NAD + reduction to form NADH, and the NADH production rate is measured at 340 nm, and the G6P activity can be reflected.

 

\u0026 nbsp; required instruments, supplies: spectrophotometer / enzyme label, desktop centrifuge, Adjustable pipette, trace quantum, mortar, ice and distilled water.

 

 

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