PFP catalytic 6-phosphate jug is converted to 1,6-di phosphate jug, which transitions to 3-phosphorched glycollydehyde under an aldehyde enzyme and phosphoric acidFurther, 3-phosphate dehydrogenase and NADH catalyzed to form 3-phosphoric acid, NAD and phosphoric acid, and the absorbance change at 340 nm reflects the activity of the PFP.

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