Cysteine (Cys) Content Assay Kit

Note: Take two or three different samples for prediction before test.

Detection equipment: Spectrophotometer/Microplate reader

Cat No: AK0581

Size: 100T/96S

Description:

Protein contains three kinds of sulfur-containing amino acids: methionine, cystine and cysteine(Cys). Cys is the only sulfur-containing amino acid containing sulfhydryl groups, which derived from methionine and could be transformed with cystine. Cys participates in the formation of protein disulfide bonds, which usually  is  a  component  of  active  centers  of  protein  and  can  provide  mercapto  groups  for  other physiological and biochemical reactions. Besides, a large amount of Cys accumulates on skin and mucosal surfaces to maintain elasticity and texture of skin and keep the activity of thiolase in the process of keratin production. It has the functions of whitening, detoxification, inflammation improvement and so on.

The phosphotungstic acid is reduced to tungsten blue by Cys, and the tungsten blue has absorption peak at 600 nm. In this kit, the content of Cys is calculated by measuring the absorbance at 600 nm.

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