Ribulose 1, 5-bisphosphate carboxylase/oxygenase(Rubisco) Assay Kit Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer

Cat No: AK0548

Size:50T/48S

Product Description:

Ribulose  1,5-bisphosphate  carboxylase/oxygenase(Rubisco)  is  a  key  enzyme  in  plant  photosynthesis, which controls the carbon dioxide fixation, and restricts the shunt of carbon into the Calvin cycle and photorespiration cycle. The activity of rubisco has direct reflect on the photosynthetic rate.

 

Rubisco  catalyzes  combination  of  one  molecule  of  ribulose- 1,5-diphosphate(RuBP)  binds  and  one molecule  of carbon  dioxide  to produce  two  molecules  of 3-phosphoglycerate  (PGA).  PGA produces glyceraldehyde-3-phosphate by the action of additional 3-phosphoglycerate kinase and glyceraldehyde-3- phosphate  dehydrogenase,  which  is  accompanied by NADH  oxidation  to  form NAD+ . NADH  has  a characteristic absorption peak at 340 nm, while NAD+  does not. In this kit, the activity of rubisco is determined by the decrease rate of NADH at 340 nm.

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