Tissue and Blood Alkaline Phosphatase(AKP/ALP) Activity Assay Kit Note: Take two or three different samples for prediction before test.

Operation Equipment: Spectrophotometer/microplate reader

Catalog Number: AK0401

Size: 100T/48S

Product Description:

AKP/ALP is a zinc-containing glycoprotease, which hydrolysis various natural and synthetic phospholipid monoester compounds in alkaline condition. AKP / ALP are widely distributed in human organs, mainly in liver.

 

In alkaline condition, AKP/ALP catalyzes hydrolysis disodium phenyl phosphate to phenol, and the phenol reacts with 4-Aminoantipyrine and potassium ferricyanide to form red quinone derivative, which can be detect absorbance at 510 nm. AKP/ALP activity can be calculated by measuring the absorbance increase rate at 510 nm.

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