Drug Names

Generic Name: Mouse Thyroid Stimulating Hormone Receptor Antibody (TRAb)ELISA Kit

Purpose

Our Mouse Thyroid Stimulating Hormone Receptor Antibody (TRAb)ELISA Kit is to assay TR-Ab levels in Mouse serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to TR-Ab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen . Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for TR is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TR-Ab and HRP conjugated TR antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TR-Ab. You can calculate the concentration of TR-Ab in the samples by comparing the OD of the samples to the standard curve.

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