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Human Angiotensinogen (AGT) ELISA Kit
Human Angiotensinogen (AGT) ELISA Kit
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  • NO.:SL0122Hu
    Sample Volume:10ul
    Wavelength:450nm
    Assay range:0.3 ng/ml - 20 ng/ml
    Sensitivity:0.08 ng/ml
    Standard:27 ng/ml
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Drug Names

Generic NameHuman Angiotensinogen (AGT) ELISA Kit

Purpose

Our Human Angiotensinogen (AGT) ELISA kit is to assay AGT levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to AGT. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for AGT is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain AGT and HRP conjugated AGT antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AGT. You can calculate the concentration of AGT in the samples by comparing the OD of the samples to the standard curve.

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