Drug Names

Generic Name：Human Short-Chain Fatty Acids (ScFA) ELISA Kit

Purpose

Our Human Short-Chain Fatty Acids (ScFA) ELISA kit is to assay ScFA levels in Human serum, plasma, culture media or any biological fluid.

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to ScFA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for ScFA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ScFA and HRP conjugated ScFA antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of ScFA. You can calculate the concentration of ScFA in the samples by comparing the OD of the samples to the standard curve.

 

1. Can you tell me what the standard in the kit is?

The reference material is a mixture of several major SCfa

 

2. What epitope on SCFA is the antibody binding to?

Antibodies are specific three-dimensional structures formed by the entire SCFA molecule along with its linking arms, and this epitope is artificially constructed

 

3. Does it quantitate all SCFA?    Or just one, say butyrate?

It is the total amount of all SCfas

 

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