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Kit Name
Generic Name:
Human Cell Division Cycle Protein 25 (CDC25) ELISA Kit
Purpose
Human Cell Division Cycle Protein 25 (CDC25) ELISA Kit to assay CDC25 levels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to CDC25. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for CDC25 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain CDC25 and HRP conjugated CDC25 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of CDC25. You can calculate the concentration of CDC25 in the samples by comparing the OD of the samples to the standard curve.
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