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Purpose
Our Human acetylcholine receptor antibody(AChRab)ELISA kit is to assay AChRab-Ablevels in Human serum, plasma, culture media or any biological fluid.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific toAChRab. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen Then a Horseradish Peroxidase (HRP)- conjugated antigen specific forAChR is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that containAChRab and HRP conjugated AChR antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of AChRab. You can calculate the concentration ofAChRabin the samples by comparing the OD of the samples to the standard curve.
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