For Research Use Only. Not for Therapeutic or Diagnostic Applications.

Product Information

Intended Use

Enzyme immunoassay for the quantitative detection of Rat HSPA5 antigen in serum, plasma, culture media, or any biological fluid.

Principle

The quantitative detection of Rat HSPA5 antigen is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Rat HSPA5. After incubation, the corresponding antigen in the sample or standards will bind to the immobilized antibody. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate is added, which binds to the antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve.

Reagents and Materials Provided

	Materials provided with the kit	96T	48T	Storage
1	Instructions for Use	1	1	R.T.
2	Adhesive films/membrane	2	2	R.T.
3	Sealed bags	1	1	R.T.
4	Microplate	1	1	2-8℃
5	Standard: 5400pg/mlpg/ml	0.5ml×1 bottle	0.5ml×1 bottle	2-8℃
6	Standard diluent	1.5ml×1 bottle	1.5ml×1 bottle	2-8℃
7	HRP-Conjugate Reagent	6ml×1 bottle	3ml×1 bottle	2-8℃
8	Sample Diluent	6ml×1 bottle	3ml×1 bottle	2-8℃
9	Chromogen Solution A	6ml×1 bottle	3ml×1 bottle	2-8℃
10	Chromogen Solution B	6ml×1 bottle	3ml×1 bottle	2-8℃
11	Stop Solution	6ml×1 bottle	3ml×1 bottle	2-8℃
12	Wash Solution Concentrate	20ml (30X*) ×1bottle	20ml (20X*) ×1bottle	2-8℃

*X denotes the product is concentrated 30 or 20 times for 96 and 48 tests, respectively

For potential hazardous substances please check the safety data sheet.

The kit allows the performance of 96 or 48 reactions, including blank and standard wells.