DESCRIPTION

Used for the quantitative determination of dsRNA concentration in biological products.

PRINCIPLE OF THE ASSAY

This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for dsRNA has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any dsRNA present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for dsRNA is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of dsRNA bound in the initial step. The color development is stopped and the intensity of the color is measured.

PRECISION

Intra-Assay Precision (Precision within an assay): <10% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <15% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

Sample		1	2	3	1	2	3
n		16	16	16	24	24	24
Standard deviation		99.7	22.3	7.8	102.0	43.3	30.3
CV \(%\)		9.8	4.7	3.5	10.4	8.6	12.6

RECOVERY

The recovery of dsRNA spiked to three different levels in cell culture supernates throughout the range of the assay was evaluated.

Sample	Dilution factor	Average (%)	Range (%)
	1:2	105	90-117

LINEARITY

To assess the linearity of the assay, cell culture supernates were spiked with high concentrations of dsRNA and diluted with the Standard Diluent to produce samples with values within the dynamic range of the assay.

Sample	Dilution factor	Average (%)	Range (%)
	1:2	91	87-96
	1:4	92	87-98
	1:8	91	91-92
	1:16	111	110-111

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)