DESCRIPTION

Used for the quantitative determination of Human Anti-Monkeypox Virus IgG (A35R) concentration in serum and plasma.

PRINCIPLE OF THE ASSAY

This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus A35R antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox Virus IgG (A35R) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox Virus IgG (A35R). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox Virus IgG (A35R) bound in the initial step. The color development is stopped and the intensity of the color is measured.

PRECISION

Intra-Assay Precision (Precision within an assay): <20% Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision. Inter-Assay Precision (Precision between assays): <20% Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

Sample		1	2	3	1	2	3
n		16	16	16	24	24	24
Mean (ng/mL)		10326.5	3001.4	736.1	9833.3	2814.0	737.1
Standard deviation		805.2	419.3	138.3	797.2	282.6	117.7
CV \(%\)		7.8	14.0	18.8	8.1	10.0	16.0

RECOVERY

The recovery of Human Anti-Monkeypox virus IgG (A35R) spiked to three different levels in human serum samples throughout the range of the assay was evaluated.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=4)	1:10	105	82-120
	1:20	107	89-119

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of Human Anti-Monkeypox virus IgG (A35R) and diluted with the Standard Diluent to produce samples with values within the dynamic range of the assay.

Sample	Dilution factor	Average (%)	Range (%)
Human serum (n=4)	1:2	95	72-119
	1:4	97	83-116
	1:8	105	95-125
	1:16	98	81-129

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)