Product Name	Recombinant Rabbit Anti-CPV-2 Monoclonal Antibody
Cat No.	SLA0676M
Chinese Name	犬细小病毒VP2单克隆抗体
Host Species	Mouse
Isotype	IgG2a
Clonality	Monoclonal
Clone No.	9F8
Applications	ELISA () Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant CPV Capsid protein VP2
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	65 kDa
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.