Product Name	Recombinant Rabbit Anti-NCAM1 Monoclonal Antibody
Cat No.	SLA0902M
Chinese Name	神经细胞粘附分子1重组鼠单抗
Alias	CD56; MSK39; NCAM; E-NCAM; NCAM-1; N-CAM; N-CAM-1; NCAM-C; NCAMC; NCAM1_HUMAN; NCAM1; NCAM1_MOUSE; NCAM1_RAT; neural cell adhesion molecule 1
Host Species	Mouse
Isotype	IgG1
Clonality	Monoclonal
Clone No.	3F9
React Species	Human
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human CD56
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	95 kDa
Subcellular Location	Membrane
Gene ID	4684
Swiss-Prot	P13591
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded (Human appendix); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM/CD56) Monoclonal Antibody, Unconjugated (SLA0902M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Figure 2. Paraformaldehyde-fixed, paraffin embedded (Human renal cell carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (NCAM/CD56) Monoclonal Antibody, Unconjugated (SLA0902M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Figure 3. Paraformaldehyde-fixed, paraffin embedded Human appendix; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NCAM/CD56 Monoclonal Antibody, Unconjugated (SLA0902M) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green, SL0296G-BF488), DAPI (blue, C02-04002) was used to stain the cell nuclei.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.