Product Name	Recombinant Rabbit Anti-ATM Monoclonal Antibody
Cat No.	SLA2891M
Chinese Name	毛细血管扩张性共济失调症突变蛋白重组兔单抗
Alias	AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1; C030026E19Rik; ATM_HUMAN; ATM; Ataxia telangiectasia mutated (A-T mutated); 2.7.11.1; ATM_MOUSE; Ataxia telangiectasia mutated homolog (A-T mutated homolog); THIL_RAT; Acat1; Acetoacetyl-CoA thiolase; 2.3.1.9; ATM serine/threonine kinase; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutated; TEL1, telomere maintenance 1, homolog (S. cerevisiae)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3H2
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human ATM
Purification	affinity purified by Protein A
Predicted MW.	370 kDa
Observed MW.	350 kDa
Subcellular Location	N/A
Gene ID	472
Swiss-Prot	Q13315
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation, apoptosis, and response to DNA damage repair (DNA damage caused by such things as ionizing irradiation activates ATM kinase). The C terminal region has extensive homology to the catalytic domains of Phosphatidylinositol 3 kinases (PI3 kinases).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ATM monoclonal antibody, unconjugated (SLA2891M) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min

Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATM Monoclonal Antibody, Unconjugated (SLA2891M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining

Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ATM Monoclonal Antibody, Unconjugated (SLA2891M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining

Figure 4. The NCCIT (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ATM antibody (SLA2891M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.