Product Name	Recombinant Rabbit Anti-PNKP Monoclonal Antibody
Cat No.	SLA2806M
Chinese Name	多聚合苷酸激酶3磷酸化酶重组兔单抗
Alias	AOA4; CMT2B2; EIEE10; MCSZ; PNK; PNKP_HUMAN; PNKP; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase; polynucleotide kinase 3'-phosphatase; bifunctional polynucleotide phosphatase/kinase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4C9
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PNKP
Purification	affinity purified by Protein A
Predicted MW.	57 kDa
Observed MW.	60 kDa
Subcellular Location	Nucleus
Gene ID	11284
Swiss-Prot	Q96T60
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PNKP monoclonal antibody, unconjugated (SLA2806M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PNKP Monoclonal Antibody, Unconjugated (SLA2806M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PNKP Monoclonal Antibody, Unconjugated (SLA2806M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PNKP Monoclonal Antibody, Unconjugated (SLA2806M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.