Product Name	Recombinant Rabbit Anti-ADAMTS5 Monoclonal Antibody
Cat No.	SLA0986M
Chinese Name	软骨蛋白聚糖重组兔单抗
Alias	ADAM-TS 11; ADAM-TS 5; ADAM-TS5; ADAMTS-11; ADAMTS-5; ADAMTS11; ADMP-2; 9530092O11Rik; ADAMTS1; ASMP-2; ATS5_HUMAN; ADAMTS5; A disintegrin and metalloproteinase with thrombospondin motifs 11 (ADAM-TS 11 | ADAMTS-11); Aggrecanase-2; 3.4.24.-; ADMP2; ATS5_MOUSE; Implantin; ADAM metallopeptidase with thrombospondin type 1 motif 5; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	3B5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human ADAMTS5 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	102 kDa
Observed MW.	73 kDa
Gene ID	11096
Swiss-Prot	Q9UNA0
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and functions as an aggrecanase that cleaves aggrecan, a major proteoglycan of cartilage, and may mediate cartilage destruction in osteoarthritis. [provided by RefSeq, Feb 2016]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with ADAMTS5 monoclonal antibody, unconjugated (SLA0986M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Osteosarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAMTS5 Monoclonal Antibody, Unconjugated (SLA0986M) at 1:400 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Glioblastoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAMTS5 Monoclonal Antibody, Unconjugated (SLA0986M) at 1:400 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAMTS5 Monoclonal Antibody, Unconjugated (SLA0986M) at 1:400 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ADAMTS5 Monoclonal Antibody, Unconjugated (SLA0986M) at 1:500 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.