Product Name	Recombinant Rabbit Anti-FAP Monoclonal Antibody
Cat No.	SLA2895M
Chinese Name	成纤维细胞激活蛋白α重组兔单抗
Alias	DPPIV; FAPA; FAPalpha; SIMP; SEPR_HUMAN; FAP; 170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha (FAPalpha); Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Serine integral membrane protease (SIMP); Surface-expressed protease (Seprase); 3.4.21.26; SEPR_MOUSE; fibroblast activation protein alpha; fibroblast activation protein, alpha; seprase
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4C4
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human FAP
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	88 kDa
Observed MW.	90 kDa
Subcellular Location	Cytoplasm
Gene ID	2191
Swiss-Prot	Q12884
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with FAP monoclonal antibody, unconjugated (SLA2895M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Monoclonal Antibody, Unconjugated(SLA2895M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Monoclonal Antibody, Unconjugated(SLA2895M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Monoclonal Antibody, Unconjugated(SLA2895M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with FAP Monoclonal Antibody, Unconjugated(SLA2895M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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