Product Name	Recombinant Rabbit Anti-NDUFB4 Monoclonal Antibody
Cat No.	SLA2648M
Chinese Name	NDUFB4蛋白重组兔单抗
Alias	B15; CI-B15; Gm3244; Gm3873; 0610006N12Rik; 1300010H20Rik; NDUB4_HUMAN; NDUFB4; Complex I-B15 (CI-B15); NADH-ubiquinone oxidoreductase B15 subunit; NDUB4_MOUSE; NADH:ubiquinone oxidoreductase subunit B4; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4 (15kD, B15); NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa; complex I B15 subunit
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11F7
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NDUFB4
Purification	affinity purified by Protein A
Predicted MW.	15 kDa
Observed MW.	15 kDa
Gene ID	4710
Swiss-Prot	O95168
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with NDUFB4 monoclonal antibody, unconjugated (SLA2648M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. 25 ug total protein per lane of various lysates (see on figure) probed with NDUFB4 monoclonal antibody, unconjugated (SLA2648M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFB4 Monoclonal Antibody, Unconjugated (SLA2648M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFB4 Monoclonal Antibody, Unconjugated (SLA2648M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NDUFB4 Monoclonal Antibody, Unconjugated (SLA2648M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.