Product Name	Recombinant Rabbit Anti-PRKCSH Monoclonal Antibody
Cat No.	SLA2410M
Chinese Name	葡萄糖苷酶2β/Glucosidase IIβ重组兔单抗
Alias	80K-H; AGE-R2; G19P1; GIIB; GIIbeta; GluIIbeta; PCLD; PCLD1; PKCSH; PLD1; VASAP-60; GLU2B_HUMAN; PRKCSH; 80K-H protein; Glucosidase II subunit beta; Protein kinase C substrate 60.1 kDa protein heavy chain (PKCSH); GLU2B_MOUSE; PRKCSH beta subunit of glucosidase II; polycystic liver disease; protein kinase C substrate 80K-H; advanced glycation end-product receptor 2; glucosidase II beta subunit; glucosidase 2 subunit beta; hepatocystin
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7D3
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Glucosidase 2 beta
Purification	affinity purified by Protein A
Predicted MW.	59 kDa
Observed MW.	75 kDa
Gene ID	5589
Swiss-Prot	P14314
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Glucosidase 2 subunit beta monoclonal antibody, unconjugated (SLA2410M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucosidase 2 subunit beta Monoclonal Antibody, Unconjugated (SLA2410M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucosidase 2 subunit beta Monoclonal Antibody, Unconjugated (SLA2410M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucosidase 2 subunit beta Monoclonal Antibody, Unconjugated (SLA2410M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Glucosidase 2 subunit beta Monoclonal Antibody, Unconjugated (SLA2410M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.