Product Name	Recombinant Rabbit Anti-PFDN5 Monoclonal Antibody
Cat No.	SLA1874M
Chinese Name	前折叠蛋白亚基5重组兔单抗
Alias	MM-1; MM1; PFD5; 1190001O17Rik; 1700010A06Rik; D15Ertd697e; Eig1; PFD5_HUMAN; PFDN5; Myc modulator 1; c-Myc-binding protein Mm-1; PFD5_MOUSE; EIG-1; prefoldin subunit 5; prefoldin 5
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11D2
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PFDN5
Purification	affinity purified by Protein A
Predicted MW.	17 kDa
Observed MW.	15 kDa
Gene ID	5204
Swiss-Prot	Q99471
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PFDN5 monoclonal antibody, unconjugated (SLA1874M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (SLA1874M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (SLA1874M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (SLA1874M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (SLA1874M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.