Product Name	Recombinant Rabbit Anti-CHEK2 Monoclonal Antibody
Cat No.	SLA1619M
Chinese Name	细胞周期检测点激酶2重组兔单抗
Alias	CDS1; CHK2; HuCds1; LFS2; PP1425; RAD53; TPDS4; hCds1; CHK2_HUMAN; CHEK2; CHK2 checkpoint homolog; Cds1 homolog (Hucds1 | hCds1); Checkpoint kinase 2; 2.7.11.1; CHK2_MOUSE; Q9R019_RAT; CHK2 (checkpoint, S.pombe) homolog; CHK2 checkpoint homolog (S. pombe)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	7D13
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Chk2
Purification	affinity purified by Protein A
Predicted MW.	61 kDa
Observed MW.	61 kDa
Gene ID	11200
Swiss-Prot	O96017
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CHEK2 monoclonal antibody, unconjugated (SLA1619M) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHEK2 Monoclonal Antibody, Unconjugated (SLA1619M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHEK2 Monoclonal Antibody, Unconjugated (SLA1619M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHEK2 Monoclonal Antibody, Unconjugated (SLA1619M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CHEK2 Monoclonal Antibody, Unconjugated (SLA1619M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.

Ordering & shipping

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