Product Name	Recombinant Rabbit Anti-CaMK-IIβ&CaMK-IIg Monoclonal Antibody
Cat No.	SLA3121M
Chinese Name	钙/钙调素依赖蛋白激酶2b/2γ重组兔单抗
Alias	CAM2; CAMK2; CAMKB; CaMKIIbeta; MRD54; CaMKII; Ck2b; KCC2B_HUMAN; CAMK2B; CaM kinase II subunit beta; CaMK-II subunit beta; 2.7.11.17; KCC2B_MOUSE; Camk2d; KCC2B_RAT; calcium/calmodulin dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; CaM-kinase II beta chain; calcium/calmodulin-dependent protein kinase type II beta chain; CaM kinase II beta subunit; proline rich calmodulin-dependent protein kinase; Camk2a; CAMK2G; CAMKA
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
React Species	Human,Mouse,Rat
Applications	IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human CaMK2 beta
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	73/63 kDa
Subcellular Location	Cytoplasm
Gene ID	816
Swiss-Prot	Q13554
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Autophosphorylation plays an important role in the regulation of the kinase activity. CaMKII is required for synaptic plasticity, as in Long Term Potentiation (LTP), a cellular model for learning and memory.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CaMKII beta/gamma Monoclonal Antibody, Unconjugated (SLA3121M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CaMKII beta/gamma Monoclonal Antibody, Unconjugated (SLA3121M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CaMKII beta/gamma Monoclonal Antibody, Unconjugated (SLA3121M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CaMKII beta/gamma Monoclonal Antibody, Unconjugated (SLA3121M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CaMKII beta/gamma Monoclonal Antibody, Unconjugated (SLA3121M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CaMKII beta/gamma Monoclonal Antibody, Unconjugated (SLA3121M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.