Product Name	Recombinant Rabbit Anti-PPM1G Monoclonal Antibody
Cat No.	SLA2814M
Chinese Name	蛋白质磷酸酶1G重组兔单抗
Alias	PPM1G_HUMAN; PPM1G; Protein phosphatase 1C; Protein phosphatase 2C isoform gamma (PP2C-gamma); Protein phosphatase magnesium-dependent 1 gamma; 3.1.3.16; PPM1C; PP2CG; PP2CGAMMA; PPP2CG; protein phosphatase, Mg2+/Mn2+ dependent 1G; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1G; PP2C, gamma; protein phosphatase 2C, gamma isoform
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	1D5
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PPM1G
Purification	affinity purified by Protein A
Predicted MW.	59 kDa
Observed MW.	72 kDa
Subcellular Location	Cytoplasm
Gene ID	5496
Swiss-Prot	O15355
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PPM1G monoclonal antibody, unconjugated (SLA2814M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPM1G Monoclonal Antibody, Unconjugated (SLA2814M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPM1G Monoclonal Antibody, Unconjugated (SLA2814M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPM1G Monoclonal Antibody, Unconjugated (SLA2814M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PPM1G Monoclonal Antibody, Unconjugated (SLA2814M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The A431 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PPM1G antibody (SLA2814M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.