Product Name	Recombinant Rabbit Anti-NSDHL Monoclonal Antibody
Cat No.	SLA2794M
Chinese Name	类固醇脱氢酶样蛋白NSDHL重组兔单抗
Alias	H105E3; SDR31E1; XAP104; Bpa; Str; NSDHL_HUMAN; NSDHL; Protein H105e3; 1.1.1.170; NSDHL_MOUSE; NAD(P) dependent 3-beta-hydroxysteroid dehydrogenase NSDHL; NAD(P) dependent steroid dehydrogenase-like; 3-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 31E, member 1; 3beta-hydroxysteroid-4alpha-carboxylate 3-dehydrogenase (decarboxylating)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	6B2
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NSDHL
Purification	affinity purified by Protein A
Predicted MW.	42 kDa
Observed MW.	40 kDa
Subcellular Location	Membrane
Gene ID	50814
Swiss-Prot	Q15738
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Catalyzes the NAD(P)+-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity).Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with NSDHL monoclonal antibody, unconjugated (SLA2794M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NSDHL Monoclonal Antibody, Unconjugated (SLA2794M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NSDHL Monoclonal Antibody, Unconjugated (SLA2794M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NSDHL Monoclonal Antibody, Unconjugated (SLA2794M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NSDHL Monoclonal Antibody, Unconjugated (SLA2794M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The PANC-1 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NSDHL antibody (SLA2794M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.