Product Name	Recombinant Rabbit Anti-MHC II Monoclonal Antibody
Cat No.	SLA2374M
Chinese Name	组织相容性抗原DRB4重组兔单抗
Alias	DR4; DRB4; HLA-DR4B; HLA-DRB; HLA-DRB4*; DRB4_HUMAN; HLA-DRB4; MHC class II antigen DRB4; major histocompatibility complex, class II, DR beta 4
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.
React Species	Human
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human HLA DRB4
Purification	affinity purified by Protein A
Predicted MW.	30 kDa
Observed MW.	27 kDa
Subcellular Location	Membrane
Gene ID	3126
Swiss-Prot	P13762
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Human purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-DRB4 Monoclonal Antibody, Unconjugated (SLA2374M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-DRB4 Monoclonal Antibody, Unconjugated (SLA2374M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-DRB4 Monoclonal Antibody, Unconjugated (SLA2374M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-DRB4 Monoclonal Antibody, Unconjugated (SLA2374M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with HLA-DRB4 Monoclonal Antibody, Unconjugated (SLA2374M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-HLA-DRB4 antibody (SLA2374M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.