Product Name	Recombinant Rabbit Anti-CNTNAP2 Monoclonal Antibody
Cat No.	SLA2329M
Chinese Name	轴突相关CNTP2蛋白重组兔单抗（少突胶质细胞）
Alias	AUTS15; CASPR2; CDFE; NRXN4; PTHSL1; 5430425M22Rik; mKIAA0868; CNTP2_HUMAN; CNTNAP2; Cell recognition molecule Caspr2; KIAA0868; CNTP2_MOUSE; contactin associated protein 2; contactin associated protein like 2
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10D1
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human CNTNAP2
Purification	affinity purified by Protein A
Predicted MW.	148 kDa
Observed MW.	170 kDa
Subcellular Location	Membrane
Gene ID	26047
Swiss-Prot	Q9UHC6
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Required for gap junction formation (Probable). Required, with CNTNAP1, for radial and longitudinal organization of myelinated axons. Plays a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Demarcates the juxtaparanodal region of the axo-glial junction.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with CNTNAP2 monoclonal antibody, unconjugated (SLA2329M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNTNAP2 Monoclonal Antibody, Unconjugated (SLA2329M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNTNAP2 Monoclonal Antibody, Unconjugated (SLA2329M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNTNAP2 Monoclonal Antibody, Unconjugated (SLA2329M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNTNAP2 Monoclonal Antibody, Unconjugated (SLA2329M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CNTNAP2 Monoclonal Antibody, Unconjugated (SLA2329M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.