Product Name	Recombinant Rabbit Anti-NUDC Monoclonal Antibody
Cat No.	SLA2070M
Chinese Name	细胞核分离基因C蛋白重组兔单抗
Alias	HNUDC; MNUDC; NPD011; SIG-92; Silg92; c15; NUDC_HUMAN; NUDC; Nuclear distribution protein C homolog; NUDC_MOUSE; Silica-induced gene 92 protein (SIG-92); NUDC_RAT; nuclear distribution C, dynein complex regulator; nuclear distribution gene C homolog (A. nidulans); nuclear distribution C homolog (A. nidulans); nudC nuclear distribution protein
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4E10
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Flow-Cyt (1:50-100) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human NUDC
Purification	affinity purified by Protein A
Predicted MW.	38 kDa
Observed MW.	45 kDa
Subcellular Location	N/A
Gene ID	10726
Swiss-Prot	Q9Y266
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Plays a role in neurogenesis and neuronal migration.Necessary for correct formation of mitotic spindles and chromosome separation during mitosis.Necessary for cytokinesis and cell proliferation.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with NUDC monoclonal antibody, unconjugated (SLA2070M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (SLA2070M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (SLA2070M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (SLA2070M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NUDC Monoclonal Antibody, Unconjugated (SLA2070M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining	Figure 6. The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NUDC antibody (SLA2070M,1:100); Isotype Control (orange): Rabbit IgG (SL0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.