Product Name	Recombinant Rabbit Anti-LGALS4 Monoclonal Antibody
Cat No.	SLA1658M
Chinese Name	Galectin 4重组兔单抗
Alias	LEG4_HUMAN; LGALS4; Gal-4; Antigen NY-CO-27; L-36 lactose-binding protein (L36LBP); Lactose-binding lectin 4; GAL4; L36LBP; galectin 4; lectin, galactoside-binding, soluble, 4
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	8A4
React Species	Human,Rat
Applications	WB (1:500-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human Galectin 4
Purification	affinity purified by Protein A
Predicted MW.	36 kDa
Observed MW.	36 kDa
Gene ID	3960
Swiss-Prot	P56470
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Galectin 4 monoclonal antibody, unconjugated (SLA1658M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 4 Monoclonal Antibody, Unconjugated (SLA1658M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 4 Monoclonal Antibody, Unconjugated (SLA1658M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 4 Monoclonal Antibody, Unconjugated (SLA1658M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 4 Monoclonal Antibody, Unconjugated (SLA1658M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Galectin 4 Monoclonal Antibody, Unconjugated (SLA1658M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.