Product Name	Recombinant Rabbit Anti-PPP2CA Monoclonal Antibody
Cat No.	SLA1230M
Chinese Name	磷酸化蛋白磷酸酶2A重组兔单抗
Alias	HJS3; NEDLBA; PP2Ac; PP2CA; PP2Calpha; RP-C; PP2Abeta; PP2CB; PP2A; D8Ertd766e; Pp2a1; Pp2a2; PP2AB_HUMAN; PPP2CB; PP2A-beta; 3.1.3.16; PP2AA_HUMAN; PPP2CA; PP2A-alpha; Replication protein C (RP-C); PP2AB_MOUSE; PP2AA_MOUSE; PP2AB_RAT; PP2AA_RAT; protein phosphatase 2 catalytic subunit alpha; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; PPP2CA | PP2A alpha (phospho-S307); p-PP2A alpha; phospho-PP2A alpha; p-PPP2CA; PPP2CA | PP2A alpha (phospho-Ser307)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	10C3
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human PP2A alpha around the phosphorylation site of S307
Purification	affinity purified by Protein A
Predicted MW.	36 kDa
Observed MW.	36 kDa
Gene ID	5515
Swiss-Prot	P62714
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PP2A alpha (Ser307) monoclonal antibody, unconjugated (SLA1230M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (SLA1230M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (SLA1230M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (SLA1230M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (SLA1230M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PP2A alpha (Ser307) Monoclonal Antibody, Unconjugated (SLA1230M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.