Product Name	Recombinant Rabbit Anti-CDKN1A Monoclonal Antibody
Cat No.	SLA0999M
Chinese Name	p21蛋白重组兔单抗
Alias	CAP20; CDKN1; CIP1; MDA-6; P21; SDI1; WAF1; p21CIP1; CDKI; mda6; p21WAF; UV96; CDN1A_HUMAN; CDKN1A; CDK-interacting protein 1; Melanoma differentiation-associated protein 6 (MDA-6); PIC1; CDN1A_MOUSE; Melanoma differentiation-associated protein; cyclin dependent kinase inhibitor 1A; cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	4D5
React Species	Human,Rat
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) ELISA (1:5000-10000) IP (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	Recombinant human p21 protein
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	18 kDa
Gene ID	1026
Swiss-Prot	P38936
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN1A | p21 Monoclonal Antibody, Unconjugated (SLA0999M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN1A | p21 Monoclonal Antibody, Unconjugated (SLA0999M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN1A | p21 Monoclonal Antibody, Unconjugated (SLA0999M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN1A | p21 Monoclonal Antibody, Unconjugated (SLA0999M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN1A | p21 Monoclonal Antibody, Unconjugated (SLA0999M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CDKN1A | p21 Monoclonal Antibody, Unconjugated (SLA0999M) at 1:200 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.