Product Name	Recombinant Rabbit Anti-PMS2 Monoclonal Antibody
Cat No.	SLA3123M
Chinese Name	肿瘤错配修复基因PMS2重组兔单抗
Alias	HNPCC4; LYNCH4; MLH4; MMRCS4; PMS-2; PMS2CL; PMSL2; PMS2_HUMAN; PMS2; DNA mismatch repair protein PMS2; PMS1 protein homolog 2; 3.1.-.-; PMS2_MOUSE; PMS1 homolog 2, mismatch repair system component; postmeiotic segregation increased (S. cerevisiae) 2; PMS2 postmeiotic segregation increased 2 (S. cerevisiae); PMS1 homolog 2, mismatch repair protein
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
React Species	Human
Applications	WB (1:500-2000) IHC-P (1:50-200) IHC-F (1:50-200) IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	103 kDa
Observed MW.	103 kDa
Gene ID	5395
Swiss-Prot	P54278
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with PMS2 monoclonal antibody, unconjugated (SLA3123M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMS2 Monoclonal Antibody, Unconjugated (SLA3123M) at 1:50 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMS2 Monoclonal Antibody, Unconjugated (SLA3123M) at 1:50 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Endometrial Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMS2 Monoclonal Antibody, Unconjugated (SLA3123M) at 1:50 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMS2 Monoclonal Antibody, Unconjugated (SLA3123M) at 1:50 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMS2 Monoclonal Antibody, Unconjugated (SLA3123M) at 1:50 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PMS2 Monoclonal Antibody, Unconjugated (SLA3123M) at 1:50 overnight at 4°C, followed by conjugation to the SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.